- Title
- Airway mucins promote immunopathology in virus-exacerbated chronic obstructive pulmonary disease
- Creator
- Singanayagam, Aran; Footitt, Joseph; Marczynski, Matthias; Radicioni, Giorgia; Cross, Michael T.; Finney, Lydia J.; Trujillo-Torralbo, Maria-Belen; Calderazzo, Maria; Zhu, Jie; Aniscenko, Julia; Clarke, Thomas B.; Molyneaux, Philip L.; Bartlett, Nathan W.; Moffatt, Miriam F.; Cookson, William O.; Wedzicha, Jadwiga; Evans, Christopher M.; Boucher, Richard C.; Kesimer, Mehmet; Lieleg, Oliver; Mallia, Patrick; Johnston, Sebastian L.
- Relation
- The Journal of Clinical Investigation Vol. 132, Issue 8, no. e120901
- Publisher Link
- http://dx.doi.org/10.1172/JCI120901
- Publisher
- American Society for Clinical Investigation
- Resource Type
- journal article
- Date
- 2022
- Description
- The respiratory tract surface is protected from inhaled pathogens by a secreted layer of mucus rich in mucin glycoproteins. Abnormal mucus accumulation is a cardinal feature of chronic respiratory diseases, but the relationship between mucus and pathogens during exacerbations is poorly understood. We identified elevations in airway mucin 5AC (MUC5AC) and MUC5B concentrations during spontaneous and experimentally induced chronic obstructive pulmonary disease (COPD) exacerbations. MUC5AC was more sensitive to changes in expression during exacerbation and was therefore more predictably associated with viral load, inflammation, symptom severity, decrements in lung function, and secondary bacterial infections. MUC5AC was functionally related to inflammation, as Muc5ac-deficient (Muc5ac–/–) mice had attenuated RV-induced (RV-induced) airway inflammation, and exogenous MUC5AC glycoprotein administration augmented inflammatory responses and increased the release of extracellular adenosine triphosphate (ATP) in mice and human airway epithelial cell cultures. Hydrolysis of ATP suppressed MUC5AC augmentation of RV-induced inflammation in mice. Therapeutic suppression of mucin production using an EGFR antagonist ameliorated immunopathology in a mouse COPD exacerbation model. The coordinated virus induction of MUC5AC and MUC5B expression suggests that non-Th2 mechanisms trigger mucin hypersecretion during exacerbations. Our data identified a proinflammatory role for MUC5AC during viral infection and suggest that MUC5AC inhibition may ameliorate COPD exacerbations.
- Subject
- airway muchins; chronic obstructive pulmonary disease; exacerbation; viral load; MUC5AC; MUC5B; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1458994
- Identifier
- uon:45554
- Identifier
- ISSN:0021-9738
- Rights
- © 2022, Singanayagam et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
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